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grna cas9 expression vector made in  (Addgene inc)


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    Structured Review

    Addgene inc grna cas9 expression vector made in
    Grna Cas9 Expression Vector Made In, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+gfp+expression+plasmid/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/bio_rxiv__64898__2026__03__27__714694-254-9-21
    Average 96 stars, based on 4086 article reviews
    grna cas9 expression vector made in - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Mitochondrial and Redox Modifications in Huntington Disease Induced Pluripotent Stem Cells Rescued by CRISPR/Cas9 CAGs Targeting
    Article Snippet: .. The sgRNA plasmid and a Cas9-GFP expression plasmid (pCas9_GFP, a gift from Kiran Musunuru, Addgene #44719) were co-transfected into hiPSCs as described previously ( ). ..

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer (https://portals. broadinstitute.org/gpp/public/analysis-tools/sgrna-design) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9- GFP expression plasmid (RRID:Addgene_79144). ..

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer ( https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design ) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9-GFP expression plasmid (RRID: Addgene_79144 ). ..

    Article Title: Pioneer activity distinguishes activating from non‐activating SOX2 binding sites
    Article Snippet: .. CRISP‐Cas9 and a sgRNA targeting the locus was cloned into a CAS9‐GFP expression plasmid (Plasmid #111596, Addgene). ..

    Article Title: Pioneer activity distinguishes activating from non-activating SOX2 binding sites.
    Article Snippet: .. CRISP-Cas9 and a sgRNA targeting the locus was cloned into a CAS9-GFP expression plasmid (Plasmid #111596, Addgene). ..

    Expressing:

    Article Title: Mitochondrial and Redox Modifications in Huntington Disease Induced Pluripotent Stem Cells Rescued by CRISPR/Cas9 CAGs Targeting
    Article Snippet: .. The sgRNA plasmid and a Cas9-GFP expression plasmid (pCas9_GFP, a gift from Kiran Musunuru, Addgene #44719) were co-transfected into hiPSCs as described previously ( ). ..

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer (https://portals. broadinstitute.org/gpp/public/analysis-tools/sgrna-design) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9- GFP expression plasmid (RRID:Addgene_79144). ..

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer ( https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design ) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9-GFP expression plasmid (RRID: Addgene_79144 ). ..

    Article Title: Pioneer activity distinguishes activating from non‐activating SOX2 binding sites
    Article Snippet: .. CRISP‐Cas9 and a sgRNA targeting the locus was cloned into a CAS9‐GFP expression plasmid (Plasmid #111596, Addgene). ..

    Article Title: Pioneer activity distinguishes activating from non-activating SOX2 binding sites.
    Article Snippet: .. CRISP-Cas9 and a sgRNA targeting the locus was cloned into a CAS9-GFP expression plasmid (Plasmid #111596, Addgene). ..

    Transfection:

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer (https://portals. broadinstitute.org/gpp/public/analysis-tools/sgrna-design) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9- GFP expression plasmid (RRID:Addgene_79144). ..

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer ( https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design ) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9-GFP expression plasmid (RRID: Addgene_79144 ). ..

    Sequencing:

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer (https://portals. broadinstitute.org/gpp/public/analysis-tools/sgrna-design) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9- GFP expression plasmid (RRID:Addgene_79144). ..

    Article Title: SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling
    Article Snippet: .. Briefly, Jurkat T cells were transfected with two guide RNAs designed using the GPP sgRNA Designer ( https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design ) to target exon 2: AATGAACTGACGGTTAATGA and exon 3: GAAACATCAAAGGAGAACGA of the SNX9 genomic DNA sequence, together with Cas9-GFP expression plasmid (RRID: Addgene_79144 ). ..

    Clone Assay:

    Article Title: Pioneer activity distinguishes activating from non‐activating SOX2 binding sites
    Article Snippet: .. CRISP‐Cas9 and a sgRNA targeting the locus was cloned into a CAS9‐GFP expression plasmid (Plasmid #111596, Addgene). ..

    Article Title: Pioneer activity distinguishes activating from non-activating SOX2 binding sites.
    Article Snippet: .. CRISP-Cas9 and a sgRNA targeting the locus was cloned into a CAS9-GFP expression plasmid (Plasmid #111596, Addgene). ..



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    Generation of KRAS knockout clones. (A) Schematic of the <t>CRISPR/Cas9</t> strategy used to generate KRAS knockout 8988T and KP4 cell lines by targeting exon 2 of KRAS . (B) Immunoblot of RAS - less mouse embryonic fibroblasts (MEFs) reconstituted with BRAF V600E , KRAS WT , HRAS WT , or NRAS WT , confirming specificity of the anti-KRAS antibody (clone 3B10-2F2). (C) Immunoblots showing absence of KRAS protein in 8988T (K275, K328) and KP4 (K22, K63) KRAS knockout clones and baseline PI3K (pAKT) and MAPK (pERK1/2) levels relative to parental cell lines. Loading control is HSP90. Images are representative of n = 3 biological replicates. (D) Bar graphs show quantified pERK/ERK and pAKT/AKT levels relative to the parental cell line (8988T or KP4) (mean ± SEM of n = 3 biological replicates) of immunoblots in ( C ). p -values of repeated measures one-way ANOVA with Tukey’s post hoc test are shown.
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    Image Search Results


    Generation of KRAS knockout clones. (A) Schematic of the CRISPR/Cas9 strategy used to generate KRAS knockout 8988T and KP4 cell lines by targeting exon 2 of KRAS . (B) Immunoblot of RAS - less mouse embryonic fibroblasts (MEFs) reconstituted with BRAF V600E , KRAS WT , HRAS WT , or NRAS WT , confirming specificity of the anti-KRAS antibody (clone 3B10-2F2). (C) Immunoblots showing absence of KRAS protein in 8988T (K275, K328) and KP4 (K22, K63) KRAS knockout clones and baseline PI3K (pAKT) and MAPK (pERK1/2) levels relative to parental cell lines. Loading control is HSP90. Images are representative of n = 3 biological replicates. (D) Bar graphs show quantified pERK/ERK and pAKT/AKT levels relative to the parental cell line (8988T or KP4) (mean ± SEM of n = 3 biological replicates) of immunoblots in ( C ). p -values of repeated measures one-way ANOVA with Tukey’s post hoc test are shown.

    Journal: bioRxiv

    Article Title: Baseline cellular state dictates the molecular impact of KRAS mutant variants in pancreatic cancer cells

    doi: 10.64898/2026.03.10.710185

    Figure Lengend Snippet: Generation of KRAS knockout clones. (A) Schematic of the CRISPR/Cas9 strategy used to generate KRAS knockout 8988T and KP4 cell lines by targeting exon 2 of KRAS . (B) Immunoblot of RAS - less mouse embryonic fibroblasts (MEFs) reconstituted with BRAF V600E , KRAS WT , HRAS WT , or NRAS WT , confirming specificity of the anti-KRAS antibody (clone 3B10-2F2). (C) Immunoblots showing absence of KRAS protein in 8988T (K275, K328) and KP4 (K22, K63) KRAS knockout clones and baseline PI3K (pAKT) and MAPK (pERK1/2) levels relative to parental cell lines. Loading control is HSP90. Images are representative of n = 3 biological replicates. (D) Bar graphs show quantified pERK/ERK and pAKT/AKT levels relative to the parental cell line (8988T or KP4) (mean ± SEM of n = 3 biological replicates) of immunoblots in ( C ). p -values of repeated measures one-way ANOVA with Tukey’s post hoc test are shown.

    Article Snippet: A single-guide RNA (sgRNA) targeting KRAS exon 2 (sequence: 5’-AATTACTACTTGCTTCCTGT-3’) was cloned into the CRISPR-Cas9 expression vector pSpCas9(BB)-2A-GFP (PX458, Addgene plasmid #48138).

    Techniques: Knock-Out, Clone Assay, CRISPR, Western Blot, Control